Chromatography Quiz Crafted by-
Akshita Verma
Lecturer
Department of Forensic Science
Jain (deemed-to-be) University, Bangalore
Let’s have a look on answers of the quiz questions.
Que 1. Chromatography, the “the color writing” was first employed
by which Russian Scientist in 1900?
a) Mikhail Tsvet
b) Archer John
Porter Martin
c) Richard
Willstatter
d) Richard
Laurence Millington Synge
Answer: a) Mikhail Tsvet
Que 2. Characteristic
feature of any form of chromatography is?
a) Use of
molecules that are soluble in water
b) Use of an inert
carrier gas
c) Use of a mobile
and a stationary phase
d) Calculation of
an Rf value for the molecules separate
Answer: c) Use of a mobile and a stationary phase
Que 3. Which of the
following is not the principle of Chromatography?
a) Adsorption
b) Absorption
c) Affinity
d) Partition
Answer: b) Absorption
Que 4. Removal of
water or other solvents through the TLC plate is called as?
a) Elution
b) Adsorption
c) Diffusion
d) Activation
Answer: d) Activation
Que 5. The
sensitivity of TLC is of the order?
a) Milligram
b) Microgram
c) Nanogram
d) Picogram
Answer: b) Microgram
Que 6. In which type
of chromatography, the stationary phase held in a narrow tube and the mobile
phase is forced through it under pressure?
a) Column
chromatography
b) Planar
chromatography
c) Liquid
chromatography
d) Gas
chromatography
Answer: a) Column chromatography
Que 7. Paper
Chromatography generally uses?
a) One phase
solvent system
b) Two phase
solvent system
c) Three phase
solvent system
d) All of the
above
Answer: a) One phase solvent system
Que 8. Which of the
following cannot be used as adsorbent in Column adsorption chromatography?
a) Magnesium oxide
b) Silica gel
c) Activated
alumina
d) Potassium
permanganate
Answer: d) Potassium permanganate
Que 9. If the mobile
phase is gas, movement of solute is determined by its?
a) Melting point
b) Boiling point
c) Volatility
d) Solubility
Answer: c) Volatility
Que 10. In reverse
phase chromatography, the stationary phase is made?
a) Polar
b) Non polar
c) Both
d) None
Answer: b) Non polar
Que 11. The process of
passing a mobile phase through a chromatography column is called as?
a) Flushing
b) Washing
c) Elution
d) Partitioning
Answer: c) Elution
Que 12. Most modern
sample injection system in HPLC is?
a) Microvolume
sampling valve
b) Syringe
injection
c) Stop flow
injection
d) All of the above
Answer: a) Microvolume sampling valve
Que 13. Which
chromatography is using a salt gradient or a pH gradient?
a) Ion exchange
chromatography
b) High Performance Liquid
Chromatography
c) Thin Layer Chromatography
d) Gas Chromatography
Answer: a) Ion exchange chromatography
Que 14. Thermostat in
HPLC regulate the temperature of
a) Solvent
Reservoir
b) Column
c) Detector
d) Sample inlet
Answer: b) Column
Que 15. The gradient
elution in HPLC is one in which the component of solvent?
a) Remains constant
b) Changes
continuously or in series of step
c) Both of the
above
d) None of the
above
Answer: b) Changes continuously or in
series of step