Chromatographic Techniques in Forensic Science Quiz Crafted By-
Prerna Patel
Introduction to Chromatographic Techniques in Forensic Science
Chromatography is the process by which compounds within a mixture are separated. This can be achieved by properties such as size, and how the compounds interact with the mobile and solid phases of chromatography. There are different types of chromatography, ranging from 2D-based (planar) methods such as Thin Layer Chromatography (TLC) and column-based methods, including Gas Chromatography (GC) and High-Performance Liquid Chromatography (HPLC).
Let's look at the questions and their answers.
Ques 1. Chromatography was first reported by:
a) Ramsey
b) Henry
c) Pascal
d) Leduce
Answer- a) Ramsey
Ques 2. Thin Layer Chromatography is______
a) Partition Chromatography
b) Electrical mobility of ionic species
c) Adsorption chromatography
d) None of the above
Answer- c) Adsorption chromatography
Ques 3. In gas chromatography, the basis for separation of the components of the volatile material is the difference in _______
a) Partition coefficients
b) Conductivity
c) Molecular weight
d) Molarity
Answer- a) Molecular weight
Ques 4. In thin layer chromatography, the stationary phase is made of _______ and the mobile phase is made of _______
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas
Answer- a) Solid, liquid
Ques 5. Which compounds are held by the stationary phase?
a) Polar compound
b) Non-Polar compound
c) a and b both
d) None of the above
Answer- a) Polar compound
Ques 6. Thermal conductivity Detector in Gas Chromatography consists of _______ identical filaments.
a) Two
b) Four
c) Six
d) Eight
Answer- b) Four
Ques 7. The most suitable solvent for thin layer chromatography/ paper chromatography of inks is:
a) N-butanol: pyridine: water (3:1:1.5)
b) Amyl alcohol: acetic acid: chloroform (6:1:2)
c) Ethanol: acetone: acetic acid (4:1:5)
d) Amyl alcohol: acetic acid: pyridine (6:1:2)
Answer- a) N-butanol: pyridine: water (3:1:1.5)
Ques 8. What is the Analyte?
a) Substance for separation
b) Substance for impurity
c) a & b
d) none of the above
Answer- a) Substance for separation
Ques 9. Which technique is also known as color writing?
a) NMR
b) Mass spectroscopy
c) Chromatography
d) All of the above
Answer- c) Chromatography
Ques 10. Chromatography is a physical method that is used to separate ________
a) Simple mixtures
b) Complex mixtures
c) Viscous mixtures
d) Metals
Answer- b) Complex mixtures