Forensic Serology Quiz Crafted by -
Dr. Kalpesh B. Solanki
Assistant Professor
School of Forensic Science & Risk Management
Rastriya Raksha University, Gujarat, India
Introduction to Forensic Serology
Forensic serology is the detection, identification, classification, and study of various bodily fluids such as blood, semen, saliva, urine, breast milk, vomit, fecal matter and perspiration, and their relationship to a crime scene. A forensic serologist may also be involved in DNA analysis and bloodstain pattern analysis. Serology testing begins with presumptive tests which gives the analyst an indication that a specific bodily fluid may be present, but cannot completely confirm its presence. Following the presumptive tests, are the confirmatory tests that confirms what the unknown substance actually is.
Let's look at the questions and their answers
Que 1. Urease, an enzyme used for testing urea in urine, breaks down urea and release:
a) Ammonia and carbon monoxide
b) Ammonia and carbonic acid
c) Calcium and carbon dioxide
d) Phosphate and carbon dioxide
Answer: a) Ammonia and carbon monoxide
Que 2. Confirmation of menstrual blood stain is done by the following method:
a) Protein marker
b) Fibrin Degradation Product (FDP)
c) Restriction enzymes
d) Iso enzyme markers
Answer: b) Fibrin Degradation Product (FDP)
Que 3. Which of the following is a pigment found in fecal matter?
a) Creatinine
b) Melanin
c) Urobilinogen
d) Porphobilinogen
Answer: c) Urobilinogen
Que 4. Short fragments of DNA labelled with radioactive tag are known as:
a) SSR’S
b) Probes
c) STR’S
d) Primer’s
Answer: b) Probes
Que 5. A technique used to separate sperm cells and non- sperm cells for DNA analysis is called:
a) QiaAmp Extraction
b) Chelex Extraction
c) Differential Extraction
d) Organic Extraction
Answer: c) Differential Extraction
Que 6. In PCR reaction, the annealing temperature depends on:
a) AT contents of sequence
b) Primer Dimer
c) Concentration of Mg used in reaction
d) GC contents of sequence
Answer: d) GC contents of sequence
Que 7. Which of the following methods is used to locate specific gene in chromosomes?
a) Dot blot technique
b) In situ hybridization
c) Colony hybridization
d) Western Blotting
Answer: b) In situ hybridization
Que 8. A cloud of brown crystals are formed in Florene test due to formation of:
a) Choline per iodide
b) Potassium iodine
c) Potassium periodide
d) Choline iodide
Answer: a) Choline per iodide
Que 9. Rocket Immunoelectrophoresis is also known as
a) Immunodiffusion
b) Crossed-over electrophoresis
c) Laurell technique
d) Ring test
Answer: c) Laurell technique
Que 10. The most commonly used staining technique contains picroindi-gocarmine (PIC) and Nuclear Fast Red dyes. It is colloquially referred to as:
a) Periodic Acid Schiff (PAS) reagent
b) Prostrate-specific antigen
c) Christmas tree stain
d) P-dimethylaminocinnamaldehyde indicator
Answer: c)Christmas tree stain
Que 11. Seminal fluid choline is originate from the:
a) Seminal vesicle
b) Sertoli’s cell
c) Leydig cell
d) Prostate
Answer: c) Leydig cell
Que 12. In saliva examination starch iodine test shows dark purple colour change, result shows:
a) Saliva present
b) Saliva absent
c) Amylase present
d) Can not conclude
Answer: b) Saliva absent
Que 13. The following is not red cell isoenzymes:
a) Adenosine deaminase
b) Glutamate pyruvate transaminase
c) Transferrin
d) Phosphoglucomutase
Answer: c) Transferrin
Que 14. The study of hair is known as
a) Lithology
b) Histology
c) Phytology
d) Trichology
Answer: d) Trichology
Que 15. In PCR reaction, the annealing temperature depends on:
a) AT contents of sequence
b) Primer Dimer
c) Concentration of Mg used in reaction
d) GC contents of sequence
Answer: d) GC contents of sequence